Questions: Inhibiting the Action of Catechol Oxidase- Mixing Table and Results ENZYME Inhibitor SUBSTRATE Absorbance 540 nm ------------------ Tube Catechol oxidase DI Water PTU 1% catechol 1 1 mL 2 ml 2 1 ml 1 ml ... 3 1 ml 1 ml 1 ml 1 ml 1.945 4 1 ml ... 1 ml 2 ml 0.158 Tubes were prepared according to the above mixing table and the absorbance at 540 nm recorded. The question being investigated is: "What type of inhibitor is PTU, competitive or noncompetitive?" The hypothesis tested is that PTU is a competitive inhibitor. 1. Explain the experimental design. What is the purpose of test tubes 1,2,3 and 4? 2. Does the data support the hypothesis? Explain your results in terms of the data.
Transcript text: 4.2 Inhibiting the Action of Catechol Oxidase-
Mixing Table and Results
\begin{tabular}{|l|c|c|c|c|c|}
\hline & ENZYME & & Inhibitor & SUBSTRATE & \begin{tabular}{l}
Absorbance \\
540 nm
\end{tabular} \\
\hline Tube & Catechol oxidase & DI Water & PTU & $1 \%$ catechol & \\
\hline 1 & 1 mL & 2 ml & & & \\
\hline 2 & 1 ml & 1 ml & & $\ldots$ & \\
\hline 3 & 1 ml & 1 ml & 1 ml & 1 ml & 1.945 \\
\hline 4 & 1 ml & $\ldots$ & 1 ml & 2 ml & 0.158 \\
\hline
\end{tabular}
Tubes were prepared according to the above mixing table and the absorbance $@ 540$ nm recorded.
The question being investigated is: "What type of inhibitor is PTU, competitive or noncompetitive?" The hypothesis tested is that PTU is a competitive inhibitor.
1. Explain the experimental design. What is the purpose of test tubes $1,2,3$ and 4 ?
2. Does the data support the hypothesis? Explain your results in terms of the data.
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