Questions: A Urease test was performed according to standard protocol, on a bacterial culture isolated from the urine of a guinea pig. After inoculation of the Urease broth, the culture was incubated, and the outcome is shown below. An uninoculated broth is included for comparison. Based on the outcome of the test, which of the following is true? The Urease test is positive. The Urease test is negative. The bacteria in the broth survive in an environment with a high concentration of urea. The pH of the broth is greater than pH 8.

A Urease test was performed according to standard protocol, on a bacterial culture isolated from the urine of a guinea pig. After inoculation of the Urease broth, the culture was incubated, and the outcome is shown below. An uninoculated broth is included for comparison. Based on the outcome of the test, which of the following is true? The Urease test is positive. The Urease test is negative. The bacteria in the broth survive in an environment with a high concentration of urea. The pH of the broth is greater than pH 8.
Transcript text: A Urease test was performed according to standard protocol, on a bacterial culture isolated from the urine of a guinea pig. After inoculation of the Urease broth, the culture was incubated, and the outcome is shown below. An uninoculated broth is included for comparison. Based on the outcome of the test, which of the following is true? The Urease test is positive. The Urease test is negative. The bacteria in the broth survive in an environment with a high concentration of urea. The pH of the broth is greater than pH 8.
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Solution

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Solution Steps

Step 1: Analyze the Urease test results

A positive urease test is indicated by a pink color change. The inoculated tube (Urease Test result) is pink. The uninoculated tube is yellow/orange.

Step 2: Interpret the results

The color change in the inoculated tube indicates the bacteria in the sample produce urease, which hydrolyzes urea to ammonia. Ammonia increases the pH of the solution.

Step 3: Determine the correct statement

The pink color signifies a positive urease test. Because ammonia is a byproduct of urea hydrolysis, a high concentration of urea allows the bacteria to survive and produce urease, increasing the pH above 8.

Final Answer: The Urease test is positive.

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