Questions: identify the main procedures involved in a western blot and the correct order in which they are performed.

Solution Steps

The first step in the Western blot process is to separate the proteins by size using electrophoresis.

The proteins are then transferred from the gel onto a thin membrane of nitrocellulose or other absorbing compound.

The proteins on the membrane are incubated with the primary antibody, which specifically binds to the target protein.

Final Answer