Questions: What is the purpose of the 1 kbp DNA ladder used in gel electrophoresis? to quantitatively measure the speed of DNA to qualitatively measure the length of DNA to qualitatively measure the intensity of the color of DNA to quantitatively measure the size of DNA

What is the purpose of the 1 kbp DNA ladder used in gel electrophoresis?
to quantitatively measure the speed of DNA
to qualitatively measure the length of DNA
to qualitatively measure the intensity of the color of DNA
to quantitatively measure the size of DNA
Transcript text: What is the purpose of the 1 kbp DNA ladder used in gel electrophoresis? to quantitatively measure the speed of DNA to qualitatively measure the length of DNA to qualitatively measure the intensity of the color of DNA to quantitatively measure the size of DNA
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Solution

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The answer is the second one: to qualitatively measure the length of DNA.

Explanation for each option:

  1. To quantitatively measure the speed of DNA: This is incorrect. The 1 kbp DNA ladder is not used to measure the speed at which DNA fragments move through the gel. The speed is influenced by factors such as the size of the DNA fragments and the gel concentration, but the ladder itself is not used for this purpose.

  2. To qualitatively measure the length of DNA: This is correct. The 1 kbp DNA ladder is used as a reference to estimate the size of DNA fragments in a sample. By comparing the bands of the sample to the bands of the ladder, researchers can determine the approximate length of the DNA fragments.

  3. To qualitatively measure the intensity of the color of DNA: This is incorrect. The ladder is not used to measure color intensity. The intensity of the bands can give some information about the quantity of DNA, but this is not the primary purpose of the ladder.

  4. To quantitatively measure the size of DNA: This is partially correct but not the best answer. While the ladder provides a reference for estimating the size of DNA fragments, the measurement is qualitative rather than quantitative. The ladder allows for an approximation rather than an exact measurement.

In summary, the primary purpose of the 1 kbp DNA ladder in gel electrophoresis is to qualitatively measure the length of DNA fragments by providing a set of known reference points.

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