The answer is (B) +1 start site.
Explanation for each option:
(A) Origin of replication: This is related to DNA replication, not transcription. It is the site where DNA replication begins.
(B) +1 start site: This is the correct answer. In transcription, the +1 start site is where RNA polymerase begins synthesizing the RNA transcript. It is the first nucleotide that is transcribed into RNA.
(C) Promoter: The promoter is a region of DNA that initiates transcription of a particular gene. It is located upstream of the +1 start site and is where RNA polymerase and transcription factors bind to start the process, but the actual synthesis of RNA begins at the +1 start site.
(D) Start codon: This is related to translation, not transcription. The start codon (usually AUG) is the first codon of a messenger RNA (mRNA) transcript that is translated by a ribosome.
(E) 5' cap: This is a modification added to the 5' end of the pre-mRNA during RNA processing in eukaryotes, not the site where transcription begins.
In summary, the synthesis of the RNA product in transcription begins at the +1 start site.