Questions: Review the experiment carried out by Bozkur's team using Nicotiana benthamiana and Phythophthora infestans. For the experiment, match the following: Normal CHUP1 gene infection with Phythophtora infestans A. standardized treatment Status of CHUP1 gene B. independent variable Chloroplasts movement to infection site C. control treatment Shutting off photosynthetic machinery in chloroplasts D. dependent variable

The answer is as follows:

A. standardized treatment $\square$ infection with Phythophtora infestans

B. independent variable $\square$ Status of CHUP1 gene

C. control treatment $\square$ Normal CHUP1 gene

D. dependent variable $\square$ Chloroplasts movement to infection site

Explanation:

• The "infection with Phythophtora infestans" is the standardized treatment applied to all samples to observe the effect of the infection.
• The "Status of CHUP1 gene" is the independent variable because it is the condition that is being manipulated in the experiment (normal vs. altered CHUP1 gene).
• The "Normal CHUP1 gene" serves as the control treatment, providing a baseline to compare the effects of the altered CHUP1 gene.
• The "Chloroplasts movement to infection site" is the dependent variable, as it is the outcome being measured in response to the changes in the CHUP1 gene status and the infection.