Questions: The primary dye in the Gram stain is: - Gram's iodine - crystal violet - ethyl alcohol (ethanol) - safranin

The answer to Question 1 is the second one: crystal violet.

Explanation for each option:

• Gram's iodine: This is not the primary dye. It is used as a mordant in the Gram staining process to fix the dye onto the cell walls.
• Crystal violet: This is the correct answer. Crystal violet is the primary dye used in the Gram stain procedure. It initially stains all cells.
• Ethyl alcohol (ethanol): This is not a dye. It is used as a decolorizing agent in the Gram stain process.
• Safranin: This is not the primary dye. It is used as a counterstain in the Gram stain procedure to stain Gram-negative bacteria after the decolorization step.

In summary, the primary dye in the Gram stain is crystal violet.