Questions: Question 3 10 pts A positive test for indole production is the formation of a red color in the Kovac's reagent added to the SIM tube. What specific enzyme is responsible for indole production? citrate lyase tryptophanase esculinase ( β-glucosidase) urease a-amylase

The answer is the second one: tryptophanase.

Explanation for each option:

1. Citrate lyase: This enzyme is involved in the breakdown of citrate into oxaloacetate and acetate. It is not related to indole production.

2. Tryptophanase: This enzyme is responsible for the production of indole. It catalyzes the conversion of tryptophan into indole, pyruvate, and ammonia. Therefore, tryptophanase is the correct answer.

3. Esculinase (β-glucosidase): This enzyme hydrolyzes esculin into esculetin and glucose. It is not involved in indole production.

4. Urease: This enzyme catalyzes the hydrolysis of urea into carbon dioxide and ammonia. It is not related to indole production.

5. α-Amylase: This enzyme breaks down starches into sugars. It is not involved in the production of indole.

In summary, tryptophanase is the specific enzyme responsible for indole production, leading to a positive test indicated by the formation of a red color in the Kovac's reagent.