Questions: Protein samples are mixed with a matrix prior to analysis. What is the function of a matrix in MALDI MS analysis? a) Mimics the proteins molecule endogenous environment b) Amplifies protein molecule mass c) Ensures ionization of protein molecules d) Accelerates MS measurements

Protein samples are mixed with a matrix prior to analysis. What is the function of a matrix in MALDI MS analysis? a) Mimics the proteins molecule endogenous environment b) Amplifies protein molecule mass c) Ensures ionization of protein molecules d) Accelerates MS measurements
Transcript text: Protein samples are mixed with a matrix prior to analysis. What is the function of a matrix in MALDI MS analysis? a) Mimics the proteins molecule endogenous environment b) Amplifies protein molecule mass c) Ensures ionization of protein molecules d) Accelerates MS measurements
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Solution

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Solution Steps

Step 1: Understanding MALDI MS Analysis

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI MS) is a technique used to analyze biomolecules, such as proteins. In this method, a matrix is mixed with the sample to facilitate the ionization process.

Step 2: Role of the Matrix in MALDI MS

The matrix in MALDI MS plays a crucial role in ensuring the ionization of protein molecules. It absorbs the laser energy and assists in the desorption and ionization of the sample molecules without fragmenting them. This is essential for the accurate analysis of large biomolecules like proteins.

Step 3: Evaluating the Options
  • a) Mimics the proteins molecule endogenous environment: This is not the primary function of the matrix in MALDI MS.
  • b) Amplifies protein molecule mass: The matrix does not amplify the mass of protein molecules.
  • c) Ensures ionization of protein molecules: This is the correct function of the matrix in MALDI MS.
  • d) Accelerates MS measurements: While the matrix may aid in the process, its primary role is not to accelerate measurements.

Final Answer

The function of a matrix in MALDI MS analysis is to ensure ionization of protein molecules. Therefore, the answer is \(\boxed{\text{c}}\).

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