Questions: You inoculated a pure culture of unknown bacteria into a tube containing thioglycollate medium to study their metabolic relationship with oxygen. The culture was incubated for an appropriate length of time at the optimal growth temperature of the organism. When you examined the culture, you observed that although the organism grew throughout the medium, the growth was denser at the surface. What would you conclude about the oxygen requirements for this unknown species? The unknown is an obligate anaerobe. The unknown is a microaerophile. The unknown is an obligate aerobe. The unknown is a facultative anaerobe.

You inoculated a pure culture of unknown bacteria into a tube containing thioglycollate medium to study their metabolic relationship with oxygen. The culture was incubated for an appropriate length of time at the optimal growth temperature of the organism. When you examined the culture, you observed that although the organism grew throughout the medium, the growth was denser at the surface. What would you conclude about the oxygen requirements for this unknown species? The unknown is an obligate anaerobe. The unknown is a microaerophile. The unknown is an obligate aerobe. The unknown is a facultative anaerobe.
Transcript text: You inoculated a pure culture of unknown bacteria into a tube containing thioglycollate medium to study their metabolic relationship with oxygen. The culture was incubated for an appropriate length of time at the optimal growth temperature of the organism. When you examined the culture, you observed that although the organism grew throughout the medium, the growth was denser at the surface. What would you conclude about the oxygen requirements for this unknown species? The unknown is an obligate anaerobe. The unknown is a microaerophile. The unknown is an obligate aerobe. The unknown is a facultative anaerobe.
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Solution

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The answer is the fourth one: The unknown is a facultative anaerobe.

Explanation for each option:

  1. The unknown is an obligate anaerobe.

    • Incorrect. Obligate anaerobes cannot tolerate oxygen and would not grow at the surface of the medium where oxygen is present.

  2. The unknown is a microaerophile.

    • Incorrect. Microaerophiles require oxygen but at lower concentrations than are present in the atmosphere. They typically grow just below the surface of the medium, not throughout it.

  3. The unknown is an obligate aerobe.

    • Incorrect. Obligate aerobes require oxygen for growth and would primarily grow at the surface where oxygen concentration is highest, not throughout the medium.

  4. The unknown is a facultative anaerobe.

    • Correct. Facultative anaerobes can grow in the presence or absence of oxygen. They grow throughout the medium but show denser growth at the surface where oxygen is more abundant, as they can utilize oxygen for more efficient energy production.

Summary: The observed growth pattern indicates that the unknown bacteria can grow in both the presence and absence of oxygen, with a preference for oxygen when it is available. Therefore, the unknown species is a facultative anaerobe.

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