Questions: Which of the following will alter analyte retention times when its chemical nature is changed? (Note: There may be more than one correct response) The LC Stationary phase The LC mobile phase

Which of the following will alter analyte retention times when its chemical nature is changed? (Note: There may be more than one correct response) The LC Stationary phase The LC mobile phase

Transcript text: Which of the following will alter analyte retention times when it's chemical nature is changed? (Note: There may be more than one correct response) The LC Stationary phase The LC mobile phase

Solution

Solution Steps

Step 1: Understanding the Question

The question asks which factors will alter analyte retention times when their chemical nature is changed in Liquid Chromatography (LC). The options given are the LC stationary phase and the LC mobile phase.

Step 2: Analyzing the LC Stationary Phase

The LC stationary phase is the material inside the column that interacts with the analyte. Changes in the chemical nature of the stationary phase can significantly affect the retention time of analytes because the interaction between the analyte and the stationary phase determines how long the analyte is retained in the column.

Step 3: Analyzing the LC Mobile Phase

The LC mobile phase is the solvent that carries the analyte through the column. Changes in the chemical nature of the mobile phase can also alter the retention time of analytes. This is because the mobile phase can affect the solubility and interaction of the analyte with the stationary phase.