Questions: What is the purpose of today's ELISA? to detect the presence What is SLE? Systemic Lupus Erythematosus. What are you trying to detect in the patient specimens? specific Why centrifuge the blood? To separate the serum form What is PBS? Phosphate - Buffered What is on the pretreated plate? to separate the b Why are controls important? for positive and Why use 37°C as the incubation temperature? 37°C is the What would occur if the incubation time was too short? ind

The purpose of today's ELISA is to detect the presence of specific antibodies or antigens in the patient specimens. ELISA (Enzyme-Linked Immunosorbent Assay) is a common laboratory technique used for this purpose.

Systemic Lupus Erythematosus (SLE) is an autoimmune disease where the body's immune system mistakenly attacks healthy tissue. It can affect the skin, joints, kidneys, brain, and other organs.

In the patient specimens, you are trying to detect specific antibodies or antigens related to SLE. This is typically done to diagnose or monitor the disease.

1. Centrifuge the blood from patients A, B, and C in 40 ml tubes.

Why centrifuge the blood? To separate the serum from the blood cells. The serum contains the antibodies or antigens that need to be tested in the ELISA.

2. Transfer the serum to new tubes and make dilutions.

What is PBS? Phosphate-Buffered Saline. It is a buffer solution commonly used in biological research to maintain a constant pH and osmolarity, which is important for maintaining the stability of the antibodies or antigens during the assay.

3. Transfer diluted specimens to the ELISA plate.

What is on the pretreated plate? The pretreated plate is typically coated with an antigen or antibody that will specifically bind to the target antibody or antigen in the patient specimens. This allows for the detection of the target molecule.

In summary, the ELISA is being used to detect specific antibodies or antigens in patient specimens related to SLE. The process involves separating serum from blood, diluting the serum, and using a pretreated plate to capture and detect the target molecules.