Questions: LOWER MAGNIFICATION HIGHER MAG - Working distance: Longer, Can use coarse-focus knob Shorter, Very short at 1,000x, Risk of grinding lens, Use fine-focus knob - Depth of field: Deeper, Easier to focus, Can use coarse-focus knob Shallow, Easy to lose focus, Use fine-focus knob - Iris diaphragm: Use less light, Close diaphragm down to increase contrast Need more light, Open diaphragm - Condenser: Lower may improve contrast Raise to base of stage, light focus - Microscope Oil: Never use oil Use immersion oil to reduce scatter, Only for 100× oil immersion objective (1,000x total magnification)

Transcript text: digital.wwnorton.com Lab 1: The Microscope Figure 1.5 Relative size of various cells. Paramecium and red blood cells are eukaryotic cells, while the remaining cells (bact) are prokaryotic cells. Question 1 of 1 In general, prokaryotic cells are much smaller than eukaryotic cells. Click or tap "True" or "False" to answer the question. True False SUMMARY \begin{tabular}{|l|l|l|} \hline & \multicolumn{1}{|c|}{ LOWER MAGNIFICATION } & \multicolumn{1}{c|}{ HIGHER MAG } \\ \hline Working distance & \begin{tabular}{l} Longer \\ Can use coarse-focus knob \end{tabular} & \begin{tabular}{l} Shorter \\ Very short at 1,000x \\ Risk of grinding lens \\ Use fine-focus knob \end{tabular} \\ \hline Depth of field & \begin{tabular}{l} Deeper \\ Easier to focus \\ Can use coarse-focus knob \end{tabular} & \begin{tabular}{l} Shallow \\ Easy to lose focus \\ Use fine-focus knob \end{tabular} \\ \hline Iris diaphragm & \begin{tabular}{l} Use less light \\ Close diaphragm down to increase \\ contrast \end{tabular} & \begin{tabular}{l} Need more light \\ Open diaphragm uf \end{tabular} \\ \hline Condenser & Lower may improve contrast & \begin{tabular}{l} Raise to base of sta \\ light focus \end{tabular} \\ \hline Microscope Oil & Never use oil & \begin{tabular}{l} Use immersion oil t \\ scatter \\ Only for 100× oil im \\ (1,000x total magni \end{tabular} \\ \hline \end{tabular}