Questions: Based on the results of the streaking method and previously inoculated plate, how can you tell that the colony was successfully isolated? a) The bacterial colony is mixed with other colonies on the surface of the agar plate with nonuniform shape and color b) It cannot be determined just by looking at the plate c) The bacterial colony is within the cluster of other colonies on the surface of the agar plate with similar shapes and colors d) The bacterial colony is deposited few millimetres away from other colonies on the surface of the agar plate with uniform, clearly defined shape and color

Based on the results of the streaking method and previously inoculated plate, how can you tell that the colony was successfully isolated? a) The bacterial colony is mixed with other colonies on the surface of the agar plate with nonuniform shape and color b) It cannot be determined just by looking at the plate c) The bacterial colony is within the cluster of other colonies on the surface of the agar plate with similar shapes and colors d) The bacterial colony is deposited few millimetres away from other colonies on the surface of the agar plate with uniform, clearly defined shape and color
Transcript text: Based on the results of the streaking method and previously inoculated plate, how can you tell that the colony was successfully isolated? a) The bacterial colony is mixed with other colonies on the surface of the agar plate with nonuniform shape and color b) It cannot be determined just by looking at the plate c) The bacterial colony is within the cluster of other colonies on the surface of the agar plate with similar shapes and colors d) The bacterial colony is deposited few millimetres away from other colonies on the surface of the agar plate with uniform, clearly defined shape and color
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Solution

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The answer is the fourth one (or D): The bacterial colony is deposited few millimetres away from other colonies on the surface of the agar plate with uniform, clearly defined shape and color.

Explanation for each option:

a) The bacterial colony is mixed with other colonies on the surface of the agar plate with nonuniform shape and color.

  • This option is incorrect because a mixed colony with nonuniform shape and color indicates contamination or the presence of multiple types of bacteria, which means the colony is not successfully isolated.

b) It cannot be determined just by looking at the plate.

  • This option is incorrect because visual inspection is a primary method for determining if a colony is isolated. A successfully isolated colony will have distinct characteristics that can be observed.

c) The bacterial colony is within the cluster of other colonies on the surface of the agar plate with similar shapes and colors.

  • This option is incorrect because a colony within a cluster of other colonies suggests that it is not isolated. Isolation means the colony should be separate from others.

d) The bacterial colony is deposited few millimetres away from other colonies on the surface of the agar plate with uniform, clearly defined shape and color.

  • This option is correct because an isolated colony will be separated from other colonies and will have a uniform, clearly defined shape and color, indicating that it is a pure culture.

Summary: To determine if a bacterial colony was successfully isolated, it should be deposited a few millimeters away from other colonies on the agar plate and should have a uniform, clearly defined shape and color. This indicates that the colony is pure and not contaminated by other bacterial species.

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