Questions: How does transcription differ in eukaryotes and bacteria? Eukaryotic transcription does not require a promoter sequence. Eukaryotic transcription requires more proteins. The initiation stage of transcription is more complex in bacteria. Bacteria have more types of RNA polymerase.

The answer is the second one: Eukaryotic transcription requires more proteins.

Explanation for each option:

1. Eukaryotic transcription does not require a promoter sequence.
   This is incorrect. Both eukaryotic and bacterial transcription require promoter sequences. Promoters are essential for the initiation of transcription as they provide a binding site for RNA polymerase and other transcription factors.

2. Eukaryotic transcription requires more proteins.
   This is correct. Eukaryotic transcription is more complex and involves a larger number of proteins, including various transcription factors and RNA polymerases. In eukaryotes, transcription initiation requires the assembly of a large pre-initiation complex, which includes general transcription factors and RNA polymerase II.

3. The initiation stage of transcription is more complex in bacteria.
   This is incorrect. The initiation stage of transcription is generally more complex in eukaryotes due to the involvement of multiple transcription factors and the need for chromatin remodeling.

4. Bacteria have more types of RNA polymerase.
   This is incorrect. Bacteria typically have a single type of RNA polymerase that synthesizes all types of RNA. In contrast, eukaryotes have three main types of RNA polymerases (I, II, and III), each responsible for transcribing different classes of genes.

In summary, the primary difference highlighted in the options is that eukaryotic transcription requires more proteins, making it more complex than bacterial transcription.