Questions: Arrange the steps of DNA replication in the order that they occur. First step Last step Answer Bank DNA ligase joins DNA fragments together. DNA polymerase synthesizes DNA. Helicase unwinds the DNA double helix. RNA primers are added. RNA primers are removed. Single-stranded DNA-binding proteins bind to each template strand.

To arrange the steps of DNA replication in the correct order, we need to understand the sequence of events that occur during this process. Here is the correct order:

1. Helicase unwinds the DNA double helix.

   • This is the first step where the enzyme helicase breaks the hydrogen bonds between the base pairs, unwinding the double-stranded DNA into two single strands.

2. Single-stranded DNA-binding proteins bind to each template strand.

   • These proteins stabilize the unwound single-stranded DNA and prevent it from re-annealing or forming secondary structures.

3. RNA primers are added.

   • Primase synthesizes short RNA primers complementary to the DNA template strands, providing a starting point for DNA polymerase.

4. DNA polymerase synthesizes DNA.

   • DNA polymerase extends the RNA primers by adding DNA nucleotides to synthesize the new DNA strand.

5. RNA primers are removed.

   • The RNA primers are removed and replaced with DNA nucleotides by DNA polymerase or another enzyme.

6. DNA ligase joins DNA fragments together.

   • This is the last step where DNA ligase seals the nicks between the newly synthesized DNA fragments (Okazaki fragments on the lagging strand), forming a continuous DNA strand.

In summary, the steps in order are:

1. Helicase unwinds the DNA double helix.
2. Single-stranded DNA-binding proteins bind to each template strand.
3. RNA primers are added.
4. DNA polymerase synthesizes DNA.
5. RNA primers are removed.
6. DNA ligase joins DNA fragments together.