Questions: Coating Buffer (1X PBS) Coating Buffer is ready to use from the manufacturer (1X Dulbecco's Phosphate Buffered Saline, Sigma Product No D 537-500 mL ) Alternatively, 1X PBS maybe prepared by diluting 100 mL of 10 X PBS with 900 mL of water Store at room temperature for up to two weeks.

Coating Buffer (1X PBS) Coating Buffer is ready to use from the manufacturer (1X Dulbecco's Phosphate Buffered Saline, Sigma Product No D 537-500 mL ) Alternatively, 1X PBS maybe prepared by diluting 100 mL of 10 X PBS with 900 mL of water Store at room temperature for up to two weeks.

Transcript text: Coating Buffer (1X PBS) Coating Buffer is ready to use from the manufacturer (1X Dulbecco's Phosphate Buffered Saline, Sigma Product No D 537-500 mL ) Alternatively, 1X PBS maybe prepared by diluting 100 mL of 10 X PBS with 900 mL of water Store at room temperature for up to two weeks.

Solution

Solution Steps

Step 1: Understanding the Problem

We need to prepare 1X PBS (Phosphate Buffered Saline) from a 10X PBS stock solution by dilution. The instructions specify diluting 100 mL of 10X PBS with 900 mL of water.

Step 2: Calculating the Dilution

To prepare 1X PBS, we use the formula for dilution: \[ C_1 V_1 = C_2 V_2 \] where \(C_1\) is the concentration of the stock solution (10X), \(V_1\) is the volume of the stock solution needed, \(C_2\) is the final concentration (1X), and \(V_2\) is the final volume.

Given: \[ C_1 = 10X, \quad C_2 = 1X, \quad V_2 = 1000 \, \text{mL} \, (100 \, \text{mL} \, \text{of} \, 10X \, \text{PBS} + 900 \, \text{mL} \, \text{of} \, \text{water}) \]

Step 3: Verifying the Dilution

We need to verify that the dilution is correct: \[ 10X \times 100 \, \text{mL} = 1X \times 1000 \, \text{mL} \] \[ 1000X \, \text{mL} = 1000X \, \text{mL} \] The equation holds true, confirming that the dilution is correct.