Questions: You were instructed to add 1.0 mL out of 5.0 mL of an undiluted sample to 99 mL of sterile diluent. Instead, you add the entire 5.0 mL to the 99 mL . A.) What was your intended dilution factor? (2 points) B.) What was your actual dilution factor? (2 points) Show your work.

To solve this problem, we need to calculate both the intended and actual dilution factors based on the volumes provided.

The intended procedure was to add 1.0 mL of the undiluted sample to 99 mL of sterile diluent.

1. Total Volume of the Mixture:

   • Sample volume = 1.0 mL
   • Diluent volume = 99 mL
   • Total volume = 1.0 mL + 99 mL = 100 mL

2. Dilution Factor Calculation:

   • Dilution factor = Total volume / Sample volume
   • Dilution factor = 100 mL / 1.0 mL = 100

Thus, the intended dilution factor was 100.

Instead, the entire 5.0 mL of the undiluted sample was added to 99 mL of sterile diluent.

1. Total Volume of the Mixture:

   • Sample volume = 5.0 mL
   • Diluent volume = 99 mL
   • Total volume = 5.0 mL + 99 mL = 104 mL

2. Dilution Factor Calculation:

   • Dilution factor = Total volume / Sample volume
   • Dilution factor = 104 mL / 5.0 mL = 20.8

Thus, the actual dilution factor was 20.8.

• The intended dilution factor was 100.
• The actual dilution factor was 20.8.