Questions: Help A student was performing the Kinyoun's acid fast staining method on a sample of bacteria. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following could explain why only blue cells were present? 1. No acid-fast bacteria are present on the slide 2. The student forgot the methylene blue staining step 3. The student forgot the carbolfuchsin staining step 4. The student forgot the acid-alcohol decolorization step Multiple Choice 1,2, and 4 1 and 2 Only 1 Onily 4 Only 2 Only 3 1 and 3

Help A student was performing the Kinyoun's acid fast staining method on a sample of bacteria. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following could explain why only blue cells were present? 1. No acid-fast bacteria are present on the slide 2. The student forgot the methylene blue staining step 3. The student forgot the carbolfuchsin staining step 4. The student forgot the acid-alcohol decolorization step Multiple Choice 1,2, and 4 1 and 2 Only 1 Onily 4 Only 2 Only 3 1 and 3
Transcript text: Help A student was performing the Kinyoun's acid fast staining method on a sample of bacteria. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following could explain why only blue cells were present? 1. No acid-fast bacteria are present on the slide 2. The student forgot the methylene blue staining step 3. The student forgot the carbolfuchsin staining step 4. The student forgot the acid-alcohol decolorization step Multiple Choice 1,2, and 4 1 and 2 Only 1 Onily 4 Only 2 Only 3 1 and 3
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Solution

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The answer is the first one (1, 2, and 4): No acid-fast bacteria are present on the slide, the student forgot the methylene blue staining step, and the student forgot the acid-alcohol decolorization step.

Explanation for each option:

  1. No acid-fast bacteria are present on the slide: If there are no acid-fast bacteria present, only non-acid-fast bacteria would be stained. Non-acid-fast bacteria would appear blue after the methylene blue counterstain.

  2. The student forgot the methylene blue staining step: If the student forgot the methylene blue staining step, the cells would not be stained blue. Therefore, this option does not explain why the cells appear blue.

  3. The student forgot the carbolfuchsin staining step: If the student forgot the carbolfuchsin staining step, the acid-fast bacteria would not be stained red, and only the non-acid-fast bacteria would be stained blue by the methylene blue counterstain.

  4. The student forgot the acid-alcohol decolorization step: If the student forgot the acid-alcohol decolorization step, the carbolfuchsin would not be washed out of the non-acid-fast bacteria, and they would appear red, not blue. Therefore, this option does not explain why the cells appear blue.

Summarizing, the correct answer is 1, 2, and 4 because these are the conditions that could lead to only blue cells being present on the slide.

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