Questions: 1. Make a well-labeled diagram of your actual gel results below. The wells are indicated by the boxes.
Discussion
1. The Introduction mentioned that there are three possible results when testing a given DNA sample. Each possible result is run in its own lane of an agarose gel. Describe and draw what these three lanes would look like.
The three possible results represent different genotypes for a particular DNA sequence. Homozygous present indicates two copies of the allele, homozygous absent indicates no copies, and heterozygous indicates one copy of the allele. The gel electrophoresis separates the DNA fragments by size. The presence or absence of the allele affects the size of the DNA fragment and thus its migration through the gel, resulting in distinct band patterns for each genotype. It is expected that different cell types from the same patient would show the same banding pattern reflecting the same genotype.
2. Based on the expected results for the gel (NOT the actual results you sketched above), what can you conclude about the genotype of the patient in her different cell types? Explain your answer.
Transcript text: 1. Make a well-labeled diagram of your actual gel results below. The wells are indicated by the boxes.
Discussion
1. The Introduction mentioned that there are three possible results when testing a given DNA sample. Each possible result is run in its own lane of an agarose gel. Describe and draw what these three lanes would look like.
The three possible results represent different genotypes for a particular DNA sequence. Homozygous present indicates two copies of the allele, homozygous absent indicates no copies, and heterozygous indicates one copy of the allele. The gel electrophoresis separates the DNA fragments by size. The presence or absence of the allele affects the size of the DNA fragment and thus its migration through the gel, resulting in distinct band patterns for each genotype. It is expected that different cell types from the same patient would show the same banding pattern reflecting the same genotype.
2. Based on the expected results for the gel (NOT the actual results you sketched above), what can you conclude about the genotype of the patient in her different cell types? Explain your answer.
Solution
Solution Steps
Step 1: Interpreting the question
The question asks for the three possible banding patterns on a gel electrophoresis representing different genotypes for a particular DNA sequence.
Step 2: Homozygous present
This genotype would show a single band at the position corresponding to the size of the DNA fragment with the allele present.
Homozygous Present
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Step 3: Homozygous absent
This genotype would show no band at the position corresponding to the DNA fragment with the allele. The lane will be empty at this location. Other regions may show bands corresponding to other sequences, but these are not relevant to this analysis.
Homozygous Absent
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Step 4: Heterozygous
This genotype would show two bands: one at the position corresponding to the DNA fragment with the allele present, and another at a different position representing the DNA fragment without the allele.
Heterozygous
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Final Answer: The three possible banding patterns
The images above represent the three different band patterns.