Questions: Which two of the following applications are best performed with a phase-contrast microscope? Detecting bacterial endospores Studying microbial motility Detecting fluorescently-labeled structures Interpreting the Gram stain

Which two of the following applications are best performed with a phase-contrast microscope? Detecting bacterial endospores Studying microbial motility Detecting fluorescently-labeled structures Interpreting the Gram stain

Solution

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The applications best performed with a phase-contrast microscope are:

  1. Studying microbial motility
  2. Detecting bacterial endospores

Explanation for each option:

  1. Studying microbial motility: The answer is correct. Phase-contrast microscopy is particularly useful for observing live cells and their movements without the need for staining, which can kill or alter the cells. This makes it ideal for studying microbial motility.

  2. Detecting bacterial endospores: The answer is correct. Phase-contrast microscopy enhances the contrast of transparent and colorless structures, such as bacterial endospores, making them more visible without staining.

  3. Detecting fluorescently-labeled structures: The answer is incorrect. Fluorescence microscopy, not phase-contrast microscopy, is specifically designed for detecting fluorescently-labeled structures.

  4. Interpreting the Gram stain: The answer is incorrect. The Gram stain is a differential staining technique that is best interpreted using bright-field microscopy, not phase-contrast microscopy.

In summary, the best applications for a phase-contrast microscope from the given options are studying microbial motility and detecting bacterial endospores.

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