Questions: Put the events in the correct order. 1. Release of P-P from the incoming nucleotide. 2. Formation of H -bond between two nucleotides. 3. O-P bond formed between two nucleotides. 2, 1, 3 3, 1, 2 1,2,3 2, 3, 1

The answer is the third one (1, 2, 3): Release of P-P from the incoming nucleotide, Formation of H-bond between two nucleotides, O-P bond formed between two nucleotides.

Explanation:

1. Release of P-P from the incoming nucleotide: This step involves the release of pyrophosphate (P-P) from the incoming nucleotide triphosphate, which provides the energy necessary for the subsequent steps.
2. Formation of H-bond between two nucleotides: After the release of pyrophosphate, the incoming nucleotide can form hydrogen bonds with the complementary base on the template strand.
3. O-P bond formed between two nucleotides: Finally, a phosphodiester bond (O-P bond) is formed between the 3' hydroxyl group of the growing DNA strand and the 5' phosphate group of the incoming nucleotide, resulting in the elongation of the DNA strand.

Summarized Answer: The correct order of events is 1, 2, 3.