Questions: What is the dependent variable of this experiment? The protein concentration (mg / mL) The number of solutions The wavelength of the light used to measure absorbance The A280

Solution Steps

The dependent variable is the variable that is being measured or tested in an experiment. In this case, the dependent variable is the one that changes in response to the independent variable.

The graph shows the relationship between protein concentration (mg/mL) and another variable. The equation given is \( y = 0.00435x + 0.0111 \), where \( y \) is the dependent variable and \( x \) is the independent variable.

From the given options:

• The protein concentration (mg/mL) is the independent variable (x-axis).
• The number of solutions is not relevant to the graph.
• The wavelength of the light used to measure absorbance is a constant parameter, not a variable in the graph.
• The \( A_{280} \) is the absorbance at 280 nm, which is typically the dependent variable in such experiments.

Final Answer